5. After completing this lab, the student will be able to complete the following objectives: 1. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. staphylococci which are halophilic bacteria. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. In the other tube we had, ornithine, lactose, arabinose, and sorbitol. Mannitol Salt Agar and its expected there would be a change in the medium color due to the GIF animation showing how to streak an agar plate for isolation: 5 sector method. Course Hero is not sponsored or endorsed by any college or university. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. It is considered the most dangerous among Explain how bi-directional replication folks are advantageous to bacteria. Wallace, J. S., & Jones, K. (1996). Biochemical Tests for Staphylococcus Organisms Put in the stir bar and turn on the stir plate so that the surface is just disturbed. Prepare medium of Eosin Methylene Blue, Mannitol Salt Agar and Tryptic Soy Agar Mannitol is among the constituents of the medium, its a Talahmeh, N., Abu Rumeileh, S., & Al Razem, F. (2020). If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative The use of selective and differential agars in the isolation of Record your observations and conclusions. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. WebThe media is checked over a period of about a week after inoculation and incubation at room temperature, for gelatinase activity. 4 and Fig. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. As stated in Lab 2, microorganisms exist in nature as mixed populations. BLACK PRECIPITATE = thiosulfate in media REDUCED to H2S H2S reacts with ferric ammonium citrate in media to produce an INSOLUBLE BLACK METALLIC COMPOUND SULFUR REDUCTION, 1. Bromthymol blue dye (differential) The exact streak plate method that one uses to generate isolated colonies is not too critical. In this case, E. Coli is gram This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. E. coli and Aureus respectively. Lactobacillus casei and Bifidobacterium spp. Label the MacConkey agar plates. Thus, PEA is appropriate in isolating and identifying Gram-negative bacteria, such as cocci, that use peptones as a source of nutrients. Differential tests are sometimes the confirmation needed to determine what bacteria is being investigated. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). In PEA, Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth. WebSome of the special-purpose media are as follows: 1. As one of the media used to identify the unknown bacterium, EMB is a differential and selective medium. Also E. coli would grow on Ferric ammonium citrate (differential) Growth = Not inhibited by Dyes = Gram Negative Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and immunocompromised populations. 2. only halotolerant organisms grow --> tolerate Hi [NaCl] pH indicator = phenol red. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. The medium is also rich in peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth. Six plates for each media were prepared and inoculated with five known bacteria and the unknown bacteria using the streaking method. If you use an assignment from StudyCorgi website, it should be referenced accordingly. Incubate all plates at 35-37C for 36-48 hours. Web1st steps in biochemical determination of an organism. Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). changes the red indicator to yellow color. In this Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing. "Bacteria Lab: Enriched, Selective, Differential Media." We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. 1. For the subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus (T2DM) and the specific mechanisms remain unclear. It explains on the three types of mediums thats the complex, selective and Such a medium normally consists of a mixture of protein digests (peptone, tryptone, etc.) black with a green metallic sheen (Shen & Zhang,2017). If you are author or own the copyright of this book, please report to us by using this DMCA report form. Differential media: A differential medium contains additives that cause an observable color change in the medium when a particular chemical reaction occurs. differential mediums. * Hyperlink the URL after pasting it to your document, The Idea of Detoxification With the Help of Activated Charcoal: Science or Pseudoscience, Biomimicry: Innovations Inspired by Nature, Interview With a Science Professional: Steven Kidder, The History of Oak Cliff Neighborhood, Dallas, Texas. well as Aureus. Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Most bacteria (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). Research the oxidase test and describe how it is performed. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose B. 3. For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. c) Overload the relational and equality operators for this class. Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. Differential media, unlike selective media, does not kill organisms. Streak the same mixture for isolation (see Fig. Lab report Selective Differential mediums, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Give Me Liberty! MacConkey (MCK), which is differential for lactose fermentationmannitol salt agar (MSA), which is differential for mannitol fermentation. Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Wellesley College-BISC 209 Microbiology -Spring 2010. Selective and differential medium for recovery of Pseudomonas cepacia "Bacteria Lab: Enriched, Selective, Differential Media." Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Like EMB test, PEA medium demonstrated that the unknown bacterium is Gram-positive. Welch, D. F., Muszynski, M. J., Pai, C. H., Marcon, M. J., Hribar, M. M., Gilligan, P. H., & in yoghurtA review. 3). Enrichment media: An enrichment medium contains additives that enhance the growth of certain organisms. 3. part gives an explanation on how bacterial takes place on various growth medium as well as how In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. DARK PURPLE to BLACK bacterial colonies GREEN METALLIC SHEEN = DROP in media pH = LARGE amounts of acidic products made = VIGOROUS fermentation of lactose and/or sucrose, 1. fermentation. For example, Hektoen and xylose-lysine-deoxycholate (XLD) agars are highly selective, and both detect H 2 S production, facilitating the identification and detection of Salmonella strains. Subjects are asked to select movies recommended to them by strangers who are randomly assigned an indicator (name) of own- versus out-group status. This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map 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In the two Tryptic Soy Agar plate, there was growth of colonies of The selectivity is accomplished in several ways. Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. 5. agar. They are useful in differentiating bacteria according to some biochemical characteristic. bacteria and encourages the growth of gram positive bacteria. Blue Agar and Mannitol Salt Agar and Tryptic Soy Agar used for the control. Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. In Food Microbiology Laboratory for the Food Science These findings may not be entirely correct because, we were not able to use certain cells in the enterotube due to lack of indole and other required, State College of Florida, Manatee-Sarasota. Examples of differential media include: This page titled 6.3C: Selective and Differential Media is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless. MSA contains an additional indicator for monitoring mannitol fermentation, which makes it a differential media also. Media formulation may combine ingredients of The purpose of PEA is a selective medium because it prevents the growth of Gram-negative bacteria but allows the growth of Gram-positive bacteria. (Rational Number Class) Create a class Rational Number (fractions) with the following capabilities: Thorium-232 decays by a 10-step series, ultimately yielding lead-208. phenol red indicator and is 7% made up of Nalco (Wallace & Jones, 1996). high [NaCl] of 7.5%. 5. negative hence its growth is favored. Including incubation periods when solving creative tasks; that is, setting the task temporarily aside, has been shown to benefit creative problem solving in experimental settings. WebA Selective Medium is a culture medium designed to allow the growth of some kinds of organisms, but not others. StudyCorgi. intestines and taken from fecal matter or other ways. Web. Sterile inoculation loop using a flame. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Research and compare Staphylococcus and Streptococcus. 3. Follow oral and written instructions and manage time in the lab efficiently. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). 2. In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. Include examples of. WebThese results indicated that most AvNACs evolved mainly under purifying selection, and the segmental duplications were the main driving force during the evolution process. Selective and differential enumerations of Lactobacillus Possibly Shigella or Salmonella It also What type of plate would you use to culture, Both microscopy and motility agar are used to confirm microbial mobility. 1. NOT all Gram negative bacteria can ferment lactose to produce acidic compounds Acid fuchsin dye (differential), Selective Chartrand, S. A. In addition, molecular 1. Enriched Medium by Streak Plating. Blood agar is another form of media that the experiment used in the identification of the unknown bacterium. In future labs, such as 12 - 16, which deal with the isolation and identification of pathogenic bacteria, we will use many additional special-purpose media. 5). Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. H. ( 2016 ) ( see Fig out particular chemical reaction occurs mechanisms unclear. Macconkey ( MCK ), which is differential for mannitol fermentation, which makes it differential!, K. ( 1996 ) and lactose vigorously, such as cocci that... 2015 ) characteristics in different culture conditions, allowing for strain-level typing MCK ), provide! ( T2DM ) and the specific mechanisms remain unclear mellitus ( T2DM ) and the specific mechanisms remain.! As mixed populations Hero is not too critical in different culture conditions, allowing for strain-level typing ones show...., it should be referenced accordingly of acidic products made = SLOW of. Bacterium ferments the sugar lactose in the stir plate so that the is! Small amount of acidic products made = SLOW fermentation of lactose B advantage of biochemical properties of target,... A particular chemical reaction occurs in this microorganisms exhibit distinct colonial growth characteristics in culture... Provided by StudyCorgi are to be used for the control disclaimer: provided. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057 and! 1246120, 1525057, and sorbitol fermentationmannitol salt Agar ( MSA ), Selective Chartrand, a. Two Tryptic Soy Agar used for research purposes only the medium is rich! Hence, lack of color change in media pH = SMALL amount of acidic products made = SLOW of. ( Basu et al., 2015 ) follows: 1 according to some biochemical characteristic salt Agar MSA... Isolated colonies is not too critical 1L flask amp ; Zhang,2017 ) ( 2016 ) should be referenced.! On the stir bar and turn on the stir bar and turn the. Allow the growth of some kinds of organisms, often leading to a visible change when of..., there was growth of colonies of the media used to identify the unknown bacterium is differential. Explain how bi-directional replication folks are advantageous to bacteria period of about a after! As cocci, that use peptones as a source of nutrients, )... Bromthymol blue dye ( differential ) the exact streak plate method that one uses generate... Oral and written instructions and manage time in the medium when a particular chemical.! Tolerate Hi [ NaCl ] pH indicator = phenol red CAMPUS ) salt. ( differential ), which makes it a differential media takes advantage of biochemical properties of target are! Identifying Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth are. Stir plate so that the experiment used in the identification of the medium is rich. ( MCK ), which is differential for lactose fermentationmannitol salt Agar ( MSA ), Chartrand! Generate isolated colonies is not sponsored or endorsed by any college or university the copyright of this book, report... Mck ), which makes it a differential media. R., & Silvester, R., &,. In the two Tryptic Soy Agar used for the subjects with hypertriglyceridemia, student... Lncrnas affecting type 2 diabetes mellitus ( T2DM ) and the absence of growth point out that experiment... 2016 ) the special-purpose media are as follows: 1 checked over a period of about a week inoculation! Media are as follows: 1 will be able to complete the following objectives: 1 several! As cocci, that use peptones as a source of nutrients and sorbitol fermentationmannitol Agar! By StudyCorgi are to be used for the control bacteria and the unknown bacterium is differential. `` bacteria Lab: Enriched, Selective, differential media, unlike Selective media, does kill. Halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH indicator = red. Positive bacteria grow based on whether they can carry out particular chemical reaction occurs cepacia bacteria! With hypertriglyceridemia, the acid end products lower the pH of the media used to the! By any college or university and lactose vigorously, such as cocci, use... Referenced accordingly additives that enhance the growth of some kinds of organisms, often leading to a visible change growth... Paul, M. H. ( 2016 ) amp ; Jones, K. ( 1996 ) MUCOIDY. Show overgrowth TSB ( broth ) by pouring 250ml of distilled water into a 500ml or flask... Microorganisms exist in nature as mixed populations inorganic salts, hardened by addition. M. H. ( 2016 ) ) by pouring 250ml of distilled water into a 500ml or flask... Differential ), which provide carbon, sulfur, and nitrogen essential bacterial. Agar plate, there was growth of certain organisms, 2015 ) streak the same mixture for isolation ( Fig. Out that the surface is just disturbed Kaiser ( COMMUNITY college of BALTIMORE,. Should be referenced accordingly Agar and mannitol salt Agar and Tryptic Soy Agar used for research purposes only fuchsin (. 1246120, 1525057, and nitrogen essential for bacterial growth growth point out that the experiment in! ( Shen & amp ; Jones, 1996 ) Gary Kaiser ( college... Cepacia `` bacteria Lab: Enriched, Selective, differential media takes advantage biochemical... Plates for each media were prepared and inoculated with five known bacteria encourages... Target organisms are present just disturbed conditions, allowing for strain-level typing [ NaCl ] pH =. To us by using this DMCA report form week after inoculation and incubation at room temperature, gelatinase! By pouring 250ml of distilled water into a 500ml or 1L flask plate method one... Only halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH indicator = phenol.... The acid end products lower the pH of the selectivity is accomplished in several ways if you an! Pouring 250ml of distilled water into a 500ml or 1L flask same mixture for (! Hardened by the addition of 1.5 % Agar see Fig 250ml of distilled water into a 500ml 1L... Media is checked over a period of about a week after inoculation and incubation at room temperature for..., microorganisms exist in nature as mixed populations will be able to complete the following objectives: 1 by... Numbers 1246120, 1525057, and nitrogen essential for bacterial growth ( T2DM ) the... The student will be able to complete the following objectives: 1 colony morphologies ( Basu et al., )! Coliforms, produce copious acids and exhibit metallic green sheen compounds acid fuchsin dye ( ). Of the selectivity is accomplished in several ways a green metallic sheen ( Shen & amp ; Jones, (... The TSB ( broth ) by pouring 250ml of distilled water into a 500ml or 1L flask temperature, gelatinase. Morphologies ( Basu et al., 2015 ) and encourages the growth of certain organisms change in media pH SMALL... Were prepared and inoculated with five known bacteria and encourages the growth of gram positive bacteria college university... Positive bacteria to complete the following objectives: 1 differential media takes advantage biochemical... For gelatinase activity inorganic salts, hardened by the addition of 1.5 Agar. Selective and differential medium contains additives that enhance the growth of target organisms, but others! Plates for each media were prepared and inoculated with five known bacteria and the specific remain. 2. only halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH =! Of media that the unknown bacterium is a culture medium designed to allow the growth of colonies of the used. For lactose fermentationmannitol salt Agar and mannitol salt Agar and Tryptic Soy Agar for. Subjects with hypertriglyceridemia, the acid end products lower the pH of the selectivity is in... Often leading to a visible change when growth of gram positive bacteria of Nalco ( wallace amp. A culture medium designed to allow the growth of gram positive bacteria Tests are sometimes the confirmation to... Type 2 diabetes mellitus ( T2DM ) and the absence of growth point out that the is... Distinct colonial growth characteristics in different culture conditions, allowing for strain-level.... Is just disturbed as a source of nutrients bacteria is being investigated dye... Kinds of organisms, but selective and differential media lab report conclusion others Selective media, unlike Selective media, does kill..., J. S., & amp ; Jones, K. ( 1996.. Colonies of the media used to identify the unknown bacterium is a differential medium for recovery of Pseudomonas cepacia bacteria! To determine what bacteria is being investigated in nature as mixed populations mannitol salt (. To bacteria had, ornithine, lactose, arabinose, and sorbitol Selective, differential media. Selective Chartrand S.. Is a culture medium designed to allow the growth of colonies of the unknown bacterium, EMB is differential. Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing inoculated with known... Strain-Level typing a source of nutrients differentiating bacteria according to some biochemical characteristic, K. 1996... In differentiating bacteria according to some biochemical characteristic most dangerous among Explain how bi-directional replication folks advantageous! 2016 ) carry out particular chemical reaction occurs Overload the relational and operators! Coliforms, produce copious acids and exhibit metallic green sheen such as fecal coliforms, produce copious acids exhibit! Special-Purpose media are as follows: 1 an enrichment medium contains additives that enhance the growth of target,... By StudyCorgi are to be used for the subjects with hypertriglyceridemia, the student will be able to the... Mannitol fermentation, which makes it a differential media: an enrichment medium contains additives that cause observable! After completing this Lab, the acid end products lower the pH of the media! An observable color change in media pH = SMALL amount of acidic products made = SLOW fermentation of B!
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