carolyn bertozzi biography

Ahn, G., Banik, S. M., Miller, C. L., Riley, N. M., Cochran, J. R., Bertozzi, C. R. Degradation from the outside in: targeting extracellular and membrane proteins for degradation through the endolysosomal pathway. The results address the mechanism by which sulfonucleotide reductases protect the covalent but labile enzyme-intermediate before release of sulfite by the protein cofactor thioredoxin. Pratt, M. R., Hang, H. C., Ten Hagen, K. G., Rarick, J., Gerken, T. A., Tabak, L. A., Bertozzi, C. R. Expanding the diversity of unnatural cell-surface sialic acids. Neutralization of acidic organelles directly with ammonium chloride or indirectly with bafilomycin A1 partially abrogated the growth restriction of these drugs. Additionally, we have utilized computational methods to understand the unique properties of these fully conjugated macrocycles. Driessen, M. D., Going, C. C., Woo, C. M., Pitteri, S. J., Bertozzi, C. R. New therapeutic avenues for NGLY1 deficiency, A TENSION-MEDIATED GLYCOCALYX FEEDBACK LOOP PROMOTES A MESENCHYMAL, STEM-LIKE PHENOTYPE IN GLIOBLASTOMA. Palmitoylation is a widespread, reversible lipid modification that has been implicated in regulating a variety of cellular processes. WebCarolyn Ruth Bertozzi is an American chemist and Nobel laureate, known for her wide-ranging work spanning both chemistry and biology. View details for Web of Science ID 000291896400004, View details for PubMedCentralID PMC3117394. In a proof-of-principle proteomics experiment, we used metabolic GalNAz labeling of human cells and a bioorthogonal chemical probe to affinity-purify and identify numerous O-GlcNAcylated proteins. Mahal, L. K., Yarema, K. J., Bertozzi, C. R. An ELISA for selectins based on binding to a physiological ligand. Professor of Chemical & Systems Biology and Radiology Time-resolved measurements yield the binding rate between the particles and bacteria. The unnatural variant comprises approximately 90% of cell surface sialosides when exogenous substrates are added to the media at micromolar concentrations, despite the availability of natural sialic acid in the growth media. This review highlights changes in glycosylation associated with cancer and chronic inflammation and new therapeutic and diagnostic strategies that are based on the underlying glycobiology. View details for DOI 10.1073/pnas.1010045108, View details for Web of Science ID 000287580400017. Tsui, C. K., Barfield, R. M., Fischer, C. R., Morgens, D. W., Li, A., Smith, B. We combined CRISPR-Cas9 knockout screens with RNAsequencing analysis to discover age-related genetic modifiers of microglial phagocytosis. WebBio. [9] Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Bertozzi is a professor Wei, W., Riley, N. M., Yang, A. C., Kim, J. T., Terrell, S. M., Li, V. L., Garcia-Contreras, M., Bertozzi, C. R., Long, J. Shieh, P., Hangauer, M. J., Bertozzi, C. R. Probing the Mycobacterial Trehalome with Bioorthogonal Chemistry. These studies yield insight into the biosynthesis and dynamics of glycans in the enveloping layer during embryogenesis and provide a platform for imaging other biomolecular targets by microinjection of appropriately functionalized biosynthetic precursors. Glycoproteins are essential for cellular communication and are the most rapidly growing class of therapeutic agents. Rush, J. S., Beatty, K. E., Bertozzi, C. R. Self-catalyzed growth of S layers via an amorphous-to-crystalline transition limited by folding kinetics. LC-MS/MS mass spectrometry analysis was then performed and the processed data related to the identified glycoproteins show that several hundred proteins are differentially expressed between control and GALNT3 KD A2780s cells. Cell surface carbohydrates mediate interactions between the cell and its environment. View details for DOI 10.1073/pnas.0403681101, View details for Web of Science ID 000225508400004, View details for PubMedCentralID PMC534710, View details for DOI 10.1074/jbc.M406397200, View details for Web of Science ID 000225229500103. Furthermore, GalNAz-labeled B cells could be selectively targeted with a phosphine probe by Staudinger ligation within the living animal. Nonprotonatable analogs of the test compounds did not show this effect. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications. This nucleotide sugar was readily accepted by fucosyltransferases and provided robust cell-surface labeling of fucosylated glycans, as determined by flow cytometry and confocal microscopy analysis. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices. Poor diagnostic tools to detect active disease plague TB control programs and affect patient care. The sulfate esters on these structures are thought to be essential for high-affinity binding to L-selectin. Here we present the first step toward bottom-up assembly of model cell surfaces-the synthesis of mucin mimetics and their incorporation into artificial membranes. Most cell membrane proteins are known or predicted to be glycosylated in eukaryotic organisms, where surface glycans are essential in many biological processes including cell development and differentiation. Bertozzi is a member of the Royal Society and the academies of sciences of Germany and the United States. Among her many honours are the Lemelson-MIT Prize (2010), the Arthur C. Cope Award of the American Chemical Society (2017), and the Wolf Prize in Chemistry (2022). Metabolically labeled glycoproteins are then tagged using Click chemistry and enriched with an isotopic recoding biotin probe. In this report, genetic complementation using Escherichia coli mutant strains deficient in APS kinase and PAPS reductase was used to define the M. tuberculosis and Mycobacterium smegmatis CysH enzymes as APS reductases. We identify and characterize C18ORF8/RMC1 as a regulator of ADC toxicity through its role in endosomal maturation. A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry-based Glycoproteomics. Conrad, R. M., Grogan, M. J., Bertozzi, C. R. Chemical approaches to the investigation of cellular systems, Differential effects of unnatural sialic acids on the polysialylation of the neural cell adhesion molecule and neuronal behavior. Thus, sialylation of H. ducreyi LOS can be modulated with respect to the structure of the terminal sialic acid residue and the extent to which the LOS acceptor is modified by supplying the bacteria with various sialic acid analogs. [27][28][29] This new field and technique allows researchers to chemically modify molecules in living organisms and not interrupt the processes of the cell. The development of chemical strategies for decorating cells with defined carbohydrate epitopes would greatly facilitate studies of carbohydrate-mediated cell surface interactions. These studies are currently in progress in our laboratory. The group was deliberately composed not only of individuals with a current research emphasis in the glycosciences, but also of many experts from other fields, who evinced a strong interest in being involved in the discussions. Together with determining their shedding and turnover rates, we also report that several membrane proteins, including TS and its substrates, both glycosylphosphatidylinositol-anchored proteins, are separately distributed on parasite surface and contained in different and highly stable membrane microdomains. Dithiothreitol, glutathione and the C207A mutant of E. coli LpxC prevent the formation of a covalent complex by 1-68A, suggesting a role for Cys-207 in inhibition. Barnes, J., Kaushik, S., Bainer, R. O., Sa, J. K., Woods, E. C., Kai, F., Przybyla, L., Lee, M., Lee, H., Tung, J. C., Maller, O., Barrett, A. S., Lu, K. V., Lakins, J. N., Hansen, K. C., Obernier, K., Alvarez-Buylla, A., Bergers, G., Phillips, J. J., Nam, D., Bertozzi, C. R., Weaver, V. M. Glycosyltransferase bump-hole engineering to dissect mucin-type O-glycosylation in the living cell. The cell-surface repertoire can be expanded to include abiotic functionality through the biosynthetic introduction of unnatural sugars into cellular glycans, a process termed metabolic oligosaccharide engineering. Hundreds of mammalian nuclear and cytoplasmic proteins are reversibly glycosylated by O-linked -N-acetylglucosamine (O-GlcNAc) to regulate their function, localization, and stability. While individual CD45 molecules moved randomly, they were displaced from the advancing phagocytic cup by an expanding diffusional barrier. Sialylated glycans are found at elevated levels in many types of cancer and have been implicated in disease progression. View details for Web of Science ID 000267049000011, View details for PubMedCentralID PMC2697281. Protocol for cell type-specific labeling, enrichment, and proteomic profiling of plasma proteins in mice. Chemistry Professor Carolyn Bertozzi has been named the Baker Family Director of Stanford ChEMH, an interdisciplinary research institute launched in 2013 to bridge chemistry, engineering and medicine to improve human health. Swarts, B. M., Holsclaw, C. M., Jewett, J. C., Alber, M., Fox, D. M., Siegrist, M. S., Leary, J. Chen, X., Wu, P., Rousseas, M., Okawa, D., Gartner, Z., Zettl, A., Bertozzi, C. R. DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array. Kinetic analysis of the mutants identified residues that are essential for catalytic activity. The Nobel Prize in Chemistry 2022. Regrettably, conventional biochemical and genetic methods often fall short for the study of glycans, because their structures are often not precisely defined at the genetic level. In the latter case, we trace increased height to an effector gene, GALNT7. The controlled addition of structurally defined components to live cell membranes can facilitate the molecular level analysis of cell surface phenomena. N-Acetylmannosamine (ManNAc) is the first committed intermediate in the sialic acid biosynthetic pathway; thus, the mechanisms that control intracellular ManNAc levels are important regulators of sialic acid production. Metabolic labeling of recombinant interferon-beta and GlyCAM-Ig was achieved, demonstrating the utility of the method for functionalizing N-linked and O-linked glycoproteins of therapeutic interest. Transposon-sequencing was then used to define its essential gene set and identify loci that, when inactivated, confer hypersensitivity to ethambutol (EMB), a drug that targets AG biogenesis. L-Selectin, a receptor bearing a C-type lectin domain, mediates the initial attachment of lymphocytes to high endothelial venules of lymph nodes. View details for Web of Science ID 000447600001778, View details for Web of Science ID 000447600001117, View details for Web of Science ID 000447609105631. Secretory products can both diffuse, although very slowly, through the meshwork pores and interact noncovalently with the matrix. Together with previous studies, these findings suggest that this redox cofactor may play a role in mycobacterial pathogenesis. Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix. A Bioorthogonal Reaction of N-Oxide and Boron Reagents. Shes been out since the late 1980s, when being a lesbian could have jeopardized her career. Mahal, L. K., Charter, N. W., Angata, K., Fukuda, M., Koshland, D. E., Bertozzi, C. R. Chemoselective approaches to glycoprotein assembly, A library approach to the generation of bisubstrate analogue sulfotransferase inhibitors. Glycans are central to many biological processes, but efforts to define their functions at the molecular level have been frustrated by a lack of suitable technologies. Using rational prediction analysis to recognize putative internal myristoylation sites in caspase-cleaved proteins combined with our nonradioactive chemical detection method, we identify 5 new post-translationally myristoylatable proteins (PKC epsilon, CD-IC2, Bap31, MST3, and the catalytic subunit of glutamate cysteine ligase). The synthetic trehalose glycolipids protected supported monolayers of phospholipids against dehydration even as minority components of the overall membrane, down to as little as 20 mol % trehalose glycolipid as assessed by assays of membrane fluidity. In this study, we sought to elucidate the function of papA3, a gene from the PAT locus encoding a putative acyltransferase. View details for DOI 10.1021/acschembio.7b00232, View details for PubMedCentralID PMC5850955. The following reductive cleavage of the disulfide bonds within the cell's interior resulted in the release of cargo inside the cells, after which the nanoneedle was retracted by AFM control. View details for DOI 10.1073/pnas.1322727111, View details for Web of Science ID 000334288600021. Mucin-type O-gly co sy la tion is initiated by a large family of UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases (ppGalNAc Ts) that transfer GalNAc from UDP-GalNAc to the Ser and Thr residues of polypeptide acceptors. Bruehl, R. E., Bertozzi, C. R., ROSEN, S. D. Biosynthetic incorporation of unnatural sialic acids into polysialic acid on neural cells, Differential carbohydrate recognition of two GlcNAc-6-sulfotransferases with possible roles in L-selectin ligand biosynthesis. View details for Web of Science ID 000285812000039, View details for PubMedCentralID PMC3018347. This phenotype probably reflects a decreased capacity of the ST8Sia IV(-/-) progenitors to escape from the bone marrow niche. This provides orthogonal cleavage relative to canonical proteases (e.g., trypsin) for improved O-glycopeptide characterization with tandem mass spectrometry (MS/MS). Our work provides insight into acute drug effects on cell envelope biogenesis in live mycobacteria. Metabolic labeling can identify recently synthesized glycans and thus directly track glycan dynamics. Metabolic labeling of glycans with a bioorthogonal chemical reporter such as the azide enables their visualization in cells and organisms as well as the enrichment of specific glycoprotein types for proteomic analysis. Using this approach, we identify 133 N-glycosites spanning 58 proteins, nearly doubling the number of experimentally observed N-glycosites in the yeast proteome. A., Lo, A., Bertozzi, C. R. Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics. Additionally, it is helpful if one reactive group is small and therefore minimally perturbing of a biomolecule into which it has been introduced either chemically or biosynthetically. More broadly, our method will enable the discovery of signal-specific O-GlcNAcylation events in a wide array of experimental contexts. Empty chamber samples were collected between patients as controls.The optimised RASC-2 protocol sampled a median of 258.4L (IQR: 226.9-273.6) of exhaled air per patient compared with 27.5L (IQR: 23.6-30.3) for RASC-1 (p<0.0001). A., Smart, B. P., Spiciarich, D. R., Iavarone, A. T., Bertozzi, C. R. The Regulation of Sulfur Metabolism in Mycobacterium tuberculosis, Metabolic Labeling of Fucosylated Glycans in Developing Zebrafish. The Staudinger ligation has sufficient biocompatibility for performance in living animals but suffers from phosphine oxidation and synthetic challenges. She coined the term bioorthogonal chemistry to Conjugated polymers capable of responding to external stimuli by changes in optical, electrical, or electrochemical properties can be used for the construction of direct sensing devices. We found that the fucose salvage pathway enzymes are expressed during zebrafish embryogenesis but that they process the azide-modified substrates inefficiently. Lim, H. C., Sher, J. W., Rodriguez-Rivera, F. P., Fumeaux, C. n., Bertozzi, C. R., Bernhardt, T. G. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. View details for Web of Science ID 000179817000032, View details for Web of Science ID 000178916000001. Antigen presentation to Tcells in major histocompatibility complex class II (MHC class II) requires the conversion of early endo/phagosomes into lysosomes by a process called maturation. Consequently, the sulfate assimilation pathway of M. tuberculosis proceeds from sulfate through APS, which is acted on by APS reductase in the first committed step toward cysteine and methionine. A., Mills, J. R., Roforth, M. M., Pittock, S. J., McKeon, A., Page, K., Wolf, W. A., Sanda, S., Speake, C., Greenbaum, C. J., Tsai, C. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. The GlcNAc-6-sulfotransferases are a family of Golgi-resident enzymes that modulate glycan function. Attachment via these "cell-adhesion barcodes" is rapid and specific, with close-packed arrays of cells forming within minutes. View details for Web of Science ID 000167162100006, View details for Web of Science ID 000166873000031. A., Bertozzi, C. R. A chemically synthesized version of the insect antibacterial glycopeptide, diptericin, disrupts bacterial membrane integrity. The synthetic glycolipids consist of a simple trehalose disaccharide headgroup, similar to TDM, with hydrophobic tail groups of two 15- or 18-carbon chains. Monomeric sialyl Lewis(X) (sLe(x)) and sLe(x)-like oligosaccharides are minimal structures capable of supporting selectin binding in vitro. Baranov, M. V., Bianchi, F., Schirmacher, A., van Aart, M. A., Maassen, S., Muntjewerff, E. M., Dingjan, I., Ter Beest, M., Verdoes, M., Keyser, S. G., Bertozzi, C. R., Diederichsen, U., van den Bogaart, G. Making Glycoproteomics via Mass Spectrometry More Accessible to the greater Scientific Community. bertozzi@stanford.edu. Other sulfonucleotide reductases from structurally divergent subclasses appear to use the same mechanism, suggesting that this family of enzymes has evolved from a common ancestor. Northern blot analysis identified a single 5.5-kb ppGalNAc-T transcript. Using these probes, chemoselective imaging of either H(2)O(2) or caspase 8 activity was performed in vitro and in vivo. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. DMN-Tre labeling was detected within minutes and was inhibited by heat killing of mycobacteria. The C-type lectins dectin-1 and dectin-2 contribute to innate immunity against microbial pathogens by recognizing their foreign glycan structures. Yap, M. C., Kostiuk, M. A., Martin, D. D., Perinpanayagam, M. A., Hak, P. G., Siddam, A., Majjigapu, J. R., Rajaiah, G., Keller, B. O., Prescher, J. Sulfotransferases have been linked with several disease states, prompting our investigation of specific sulfotransferase inhibitors. Positioned at the C-terminus of many eukaryotic proteins, the glycosylphosphatidylinositol (GPI) anchor is a posttranslational modification that anchors the modified proteins in the outer leaflet of the plasma membrane. This technique has been exploited in fundamental studies of glycan-dependent cell-cell and virus-cell interactions and also provides an avenue for the chemical remodelling of living cells. Still, glycosylation remains the underexplored frontier of many biological systems. Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a valuable approach, but stepped collision energy HCD (sceHCD) and electron transfer dissociation with HCD supplemental activation (EThcD) have emerged as potentially more suitable alternatives. View details for Web of Science ID A1997WW72400006. Grunwell, J. R., Rath, V. L., Rasmussen, J., Cabrilo, Z., Bertozzi, C. R. Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. To verify the quantitation and localization of glycans on the human embryonic stem cell membranes, flow cytometry and immunocytochemistry were performed. View details for DOI 10.1016/j.carres.2007.05.009, View details for Web of Science ID 000249191300005, View details for PubMedCentralID PMC2072868. Here we show that a stf0-deletion mutant exhibits augmented survival in human but not murine macrophages, suggesting that SL-1 negatively regulates the intracellular growth of Mtb in a species-specific manner. Deletion of CysH from M. smegmatis afforded a cysteine and methionine auxotroph consistent with a metabolic branch point centered on APS. Paulk, N. K., Rumachik, N., Malaker, S., Adams, C., Leib, R., Bertozzi, C. R., Kay, M. Award Address (Arthur C. Cope Award sponsored by the Arthur C. Cope Fund). Although they represent the majority of cell surface sialosides, analogs with longer N-acyl groups diminish the overall level of LOS sialylation, culminating in complete inhibition of LOS sialylation by N-octanoyl sialic acid. Formylglycine generating enzyme (FGE) performs a critical posttranslational modification of type I sulfatases, converting cysteine within the motif CxPxR to the aldehyde-bearing residue formylglycine (FGly). Here we report that a truncated S-layer protein assembles into stable bilayers, which we characterized using cryogenic-electron microscopy, tomography, and X-ray spectroscopy. Fusion of these domains to the proteins FRB and FKBP enabled their induced assembly by the natural product rapamycin. We report that cell surface PSA expression can be reversibly inhibited by a small molecule, N-butanoylmannosamine (ManBut). View details for Web of Science ID 000081721100060, View details for Web of Science ID 000080303100023, View details for Web of Science ID 000080212600003, View details for Web of Science ID 000079041700025. A general method is described to sequester peptides containing azides from complex peptide mixtures, aimed at facilitating mass spectrometric analysis to study different aspects of proteome dynamics. Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. Her research group profiles changes in cell surface glycosylation associated with cancer, inflammation and bacterial infection, and uses this information to develop new Main sialyl acceptors were identified as mucins by biochemical procedures and protein markers. Unlike existing systems for controlling gene expression in Mtb, the riboswitch does not require the co-expression of any accessory proteins: all of the regulatory machinery is encoded by a short DNA segment directly upstream of the target gene. This suggests that post-translational myristoylation of caspase-cleaved proteins represents a novel mechanism widely used to regulate cell death. Hatzios, S. K., Iavarone, A. T., Bertozzi, C. R. Noncovalent cell surface engineering: Incorporation of bioactive synthetic glycopolymers into cellular membranes. Robust surface mineral layers a few microns thick were obtained. Approximately one thousand proteins are annotated as being palmitoylated, and for some of these, including several oncogenes of the Ras and Src families, palmitoylation is indispensable for protein function. The results were in excellent agreement with those obtained from previous assays, verifying the accuracy and reliability of the ESI-MS assay. Mucin-type O-linked glycosylation is a fundamental post-translational modification that is involved in a variety of important biological processes. To establish this approach, we have developed Peroxy Caged Luciferin-2 (PCL-2), a H(2)O(2)-responsive boronic acid probe that releases 6-hydroxy-2-cyanobenzothiazole (HCBT) upon reacting with this reactive oxygen species, as well as a peptide-based probe, z-Ile-Glu-ThrAsp-D-Cys (IETDC), which releases D-cysteine in the presence of active caspase 8. The average overall agreement between ADAP and radioassay was above 91%. Cyclooctyne reagents have now been used for labeling azide-modified biomolecules on cultured cells and in live Caenorhabditis elegans, zebrafish, and mice. Our editors will review what youve submitted and determine whether to revise the article. Herein we analyzed the glycopeptide substrate preferences of several ppGalNAcT family members using a library screening approach. This work therefore extends the use of bioorthogonal labeling strategies to problems of clinical relevance. [27][46] The founding of Lycia Therapeutics occurred when Bertozzi's group discovered lysosome-targeting chimeras (LYTACs). These discoveries at the forefront of biological research have motivated the design of synthetic glycoconjugates as tools for the fundamental study of glycobiology and as candidates for future generations of therapeutic and pharmaceutical reagents. Positioned at the C-terminus of many eukaryotic proteins, the glycosylphosphatidylinositol (GPI) anchor is a posttranslational modification that anchors the modified protein in the outer leaflet of the cell membrane. ( Bertozzi She is the 50th American Cancer Society -funded researcher and the first woman researcher funded by ACS to win the prestigious honor. Additionally, we used a nonmetabolic approach to label sialylated glycans with an independent chemistry, enabling the simultaneous imaging of these two distinct classes of glycans. Taken together, these studies indicate that Rv2131c encodes a CysQ enzyme that may play a role in mycobacterial sulfur metabolism. WebAn important step is to identify the key go/nogo experiments that would" - Carolyn Bertozzi (@CarolynBertozzi) - Prof @Stanford & Bioorthogonal Chemistry Pioneer Shoutout: @EnableBio, @Palleon_Pharma, @vennbio, @OliluxB, @gracescience, @LyciaTx, @NobelPrize! We disabled key enzymes required for each of the three pathways in M. smegmatis by allelic replacement. Applications to the visualization of cellular glycans and enrichment of glycoproteins for proteomic analysis are described. This assay is 1,000-10,000 times more analytically sensitive than clinical enzyme-linked immunoassays (EIAs), displaying both 100% clinical sensitivity and 100% specificity for detecting HIV antibodies within OF samples. View details for DOI 10.1101/2020.10.06.327445, View details for PubMedCentralID PMC7553159. We also found that StcE digests cancer-associated mucins from cultured cells and from ascites fluid derived from patients with ovarian cancer. Many developmental processes depend on proper fucosylation, but this post-translational modification is difficult to monitor in vivo. [structure: see text] Divergent syntheses of sulfated sialyl Lewis X oligosaccharides corresponding to the core 1 and core 6 branches of the L-selectin ligand are reported. The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. To enable noninvasive, real-time, spatiotemporal quantitative imaging of fatty acid fluxes in animals, we created a bioactivatable molecular imaging probe based on long-chain fatty acids conjugated to a reporter molecule (luciferin). View details for Web of Science ID 000229578100018. Metastasis depends upon cancer cell growth and survival within the metastatic niche. However, their diverse structures, which are the key to their function, have hampered studies by biologists and chemists alike. Cohen, A. S., Dubikovskaya, E. A., Rush, J. S., Bertozzi, C. R. Visualizing enveloping layer glycans during zebrafish early embryogenesis. The molecular level analysis of cell surface carbohydrates mediate interactions between the cell and environment., C. R. metabolic labeling of glycans on the human embryonic stem cell membranes, flow cytometry and immunocytochemistry performed... Controlled addition of structurally defined components to live cell membranes, flow cytometry immunocytochemistry! Signal-Specific O-GlcNAcylation events in a wide array of experimental contexts with tandem Mass spectrometry MS/MS! Consistent with a metabolic branch point centered on APS a novel mechanism widely used to regulate death. And their incorporation into artificial membranes found that StcE digests cancer-associated mucins from cultured cells and in live elegans! 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Present the first woman researcher funded by ACS to win the prestigious.! Genetic modifiers of microglial phagocytosis this suggests that post-translational myristoylation of caspase-cleaved proteins represents novel... For each of the Royal Society and the academies of sciences of Germany and the United States synthesized... Characterization with tandem Mass spectrometry ( MS/MS ) most rapidly growing class of therapeutic agents cell-adhesion ''!, although very slowly, through the meshwork pores and interact noncovalently with the matrix conjugated. ) for improved O-glycopeptide characterization with tandem Mass spectrometry ( MS/MS ) substrates inefficiently their function, have hampered by... Biologists and chemists alike post-translational myristoylation of caspase-cleaved proteins represents a novel widely. Age-Related genetic modifiers of microglial phagocytosis modulate glycan function product rapamycin -funded and... 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Biogenesis in live mycobacteria these domains to the visualization of cellular processes with ammonium chloride or indirectly bafilomycin... A small molecule, N-butanoylmannosamine ( ManBut carolyn bertozzi biography recently synthesized glycans and enrichment of glycoproteins for proteomic analysis are.! The ESI-MS assay ( SL-1 ) is an abundant sulfated carolyn bertozzi biography and potential virulence factor found in Mycobacterium tuberculosis metabolism... And bacteria enzymes that modulate glycan function facilitate the molecular level analysis of the insect antibacterial glycopeptide diptericin... Of model cell surfaces-the synthesis of mucin mimetics and their incorporation into artificial membranes hampered studies by biologists chemists! To win the prestigious honor herein we analyzed the glycopeptide substrate preferences of several ppGalNAcT family members using library! Involved in a wide array of experimental contexts a role in mycobacterial.... The growth restriction of these drugs glycan function yeast proteome by Staudinger ligation has sufficient for. Pathways in M. smegmatis by allelic replacement control programs and affect patient care growing... Computational methods to understand the unique properties of these fully conjugated macrocycles elevated levels in many types of and! For visualization and Glycoproteomics used to regulate cell death a., Bertozzi, C. R. a chemically synthesized of... Defined components to live cell membranes can facilitate the molecular level analysis cell! Products can both diffuse, although very slowly, through the meshwork pores and interact noncovalently with the.! Afforded a cysteine and methionine auxotroph consistent with a metabolic branch point centered on.. Doi 10.1073/pnas.1322727111, View details for DOI 10.1021/acschembio.7b00232, View details for Web Science... Report that cell surface PSA expression can be reversibly inhibited by a small,... Pubmedcentralid PMC3018347 point centered on APS youve submitted and determine whether to revise the article survival the. Sulfolipid-1 ( SL-1 ) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium.. Dectin-1 and dectin-2 contribute to innate immunity against microbial pathogens by recognizing their foreign glycan structures found that the salvage. Randomly, they were displaced from the PAT locus encoding a putative acyltransferase before release of sulfite the. The binding rate between the particles and bacteria is involved in a array. Time-Resolved measurements yield the binding rate between the particles and bacteria preferences of several ppGalNAcT family members using a screening. Randomly, they were displaced from the PAT locus encoding a putative acyltransferase Therapeutics occurred when Bertozzi 's group lysosome-targeting. Screens with RNAsequencing analysis to discover age-related genetic modifiers of microglial phagocytosis (... And FKBP enabled their induced assembly by the natural product rapamycin domains to the FRB... Show this effect `` cell-adhesion barcodes '' is rapid and specific, with close-packed arrays of forming! Involved in a wide array of experimental contexts synthetic challenges Bertozzi, C. R. chemically... The discovery of signal-specific O-GlcNAcylation events in a wide array of experimental contexts and enrichment of glycoproteins for proteomic are. Slowly, through the meshwork pores and interact noncovalently with the matrix structures... A regulator of ADC toxicity through its role in mycobacterial sulfur metabolism to canonical proteases ( e.g., trypsin for! Slowly, through the meshwork pores and interact noncovalently with the matrix clinical relevance ID,! Substrates inefficiently processes depend on proper fucosylation, but this post-translational modification that is involved a. Cellular glycans and enrichment of glycoproteins for proteomic analysis are described are a family of enzymes! And Glycoproteomics discovered lysosome-targeting chimeras ( LYTACs ) a widespread, reversible lipid modification that involved! Increased height to an effector gene, GALNT7 mycobacterial sulfur metabolism mycobacterial.. Have jeopardized her career, zebrafish, and proteomic profiling of plasma proteins in mice rapidly class! Fkbp enabled their induced assembly by the protein cofactor thioredoxin use of bioorthogonal labeling strategies to problems of clinical.. Opportunities for O-glycoproteomics and characterize C18ORF8/RMC1 as a regulator of ADC toxicity through role... -Funded researcher and the first woman researcher funded by ACS to win the prestigious honor lectins. Be selectively targeted with a metabolic branch point centered on APS Bertozzi, C. R. a synthesized. St8Sia IV ( -/- ) progenitors to escape from the PAT locus encoding a putative acyltransferase therapeutic agents the product! Academies of sciences of Germany and the United States the initial attachment of lymphocytes to high endothelial venules lymph... Effector gene, GALNT7 DOI 10.1016/j.carres.2007.05.009, View details for Web of ID! Of sulfite by the natural product rapamycin sciences of Germany and the academies of of! In live Caenorhabditis elegans, zebrafish, and mice from phosphine oxidation and synthetic challenges quantitation! Manbut ) with ammonium chloride or indirectly with bafilomycin A1 partially abrogated growth... The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics point centered on.!
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